However, in the undifferentiated gastric
carcinoma cell line AGS, which lacks E-cadherin expression, PKM2 promoted cell migration and invasion. Immunohistochemical analyses showed that the levels of E-cadherin expression, ERK1/2 phosphorylation, and cytoplasmic PKM2 expression were correlated with each other. Conclusion: PKM2 may play different roles in differently differentiated gastric cancer cell types, and this finding would be consistent with the previous clinical research. The results of our study reveal an important link between PKM2 and E-cadherin during EGFR-stimulated gastric cancer cell motility and invasion. Key Word(s): 1. PKM2; 2. EGF/EGFR; 3. gastric cancer; Presenting Author: JUNBO Selleckchem Decitabine HONG Additional Authors: WEI ZUO, ANJIANG WANG, NONGHUA LV Corresponding Author: JUNBO HONG, NONGHUA LV Affiliations: Hospital; hospital Objective: To determine the prevalence of intestinal metaplasia (IM) and the associated risk factors in patients with concomitant gastric and duodenal
ulcers (CGDU). Methods: Consecutive patients who underwent esophagogastroduodenal endoscopy Selleckchem AZD6244 were retrospectively screened and those presenting with endoscopically CGDU (co-existence of ulcers in both the stomach and duodenum) were further evaluated for the prevalence, demographic, endoscopic and clinical characteristics, and H. pylori infection and associations of these factors with IM. Patients with GC, dysplasia, a history of anti-H. pylori therapy and treatment with NSAIDs, H2-receptor antagonists selleck chemical or proton pump inhibitors were excluded. Results: Out of an overall
consecutive 204073 cases, 2397 (1.2%) were diagnosed with CGDU; 248 patients were excluded and thus 2149 cases (1610 males and 539 females, with a mean (±SD) age of 46.0 ± 13.5 years) were included in study. IM was observed in 180 (8.4%) patients; mild, moderate and severe grades were observed in 153 (85.0%), 26 (14.4%) and one (0.6%), respectively. Multivariate analysis identified that age of 50 years (OR = 2.606, 95%CI: 1.889–3.597, 2 = 34.000, P < 0.001), GU at the gastric incisura (OR = 2.644, 95%CI: 1.926–3.630, 2 = 36.142, P < 0.001), and H. pylori infection (OR = 2.338, 95%CI: 1.573–3.474, 2 = 17.648, P < 0.001) were independent risk factors for the development of IM. In addition, moderate/severe IM was more frequently detected in males than in females (18.8% vs. 5.8%, (OR = 3.769, 95%CI: 1.083–13.121, 2 = 4.887, P = 0.036). However, upper gastrointestinal symptoms, ulcer size and the ulcer sites in gastric antrum, gastric corpus and duodenum were not predictive factors for IM. Conclusion: CGDU is observed in approximately 1.2% of patients in China. IM occurs in 8.4% of patient with CGDU. H. pylori infection, age of ≥50 years, and ulceration at gastric incisura are independent risk factors for IM in patient with CGDU, whereas male gender is more prone to moderate/severe IM than females. Key Word(s): 1. H.