IAsys is based on an optical measurement technique that probes the thickness and dielectric constant of thin films at a cuvette surface [4-8]. QCM measures the changes in acoustic thickness or mechanical resonance properties of a thin film deposited on a metal electrode (e.g. gold, silver, and copper, etc.) [9-11]. As surface analytical tools, each of IAsys and QCM has its own specific features, weaknesses, and assumptions inherent in data collection and analysis. Combined IAsys and QCM data collection and analysis allow one to take the advantage of the features, to test the validity of the assumptions and to gain a better understanding of a specific interfacial reaction. Both the devices have been widely used for biological analysis, clinical diagnosis, and environmental monitoring [12-13].

The liquid cell configuration of the two devices makes them suitable for real-time study of bioaffinity reactions in relevant solution conditions of temperature, flow rate, pH and ionic strength [14-15]. In the recent years, the applications of IAsys and QCM for biological analyses have been reported increasingly, including immunoassay assay of BSA [16], enterotoxin Entinostat detection [17], enzymatic analysis for hydrogen peroxide quantification [18], and blood plasma coagulation determination [19].Paeoniae Radix 801 (P. radix 801), a component of Radix Paeonia Rubra, is mainly composed of propyl gallate. It is one of the active ingredients of the blood-activating and stasis-eliminating traditional Chinese medicine (TCM) [20]. By inhibiting the formation of the blood platelet Thromboxane A2 (TXA2), P.

radix 801 has many pharmacological functions, such as accelerating the formation of artery inner prostaglandin (PGI2) [21], inhibiting the formation of thrombus, resisting blood platelet aggregation [22], etc. It has been applied to cure coronary heart disease, angina, etc. [23, 24]. However, there is no detailed research so far on the action mechanism and dynamics of P. radix 801, limiting its further applications. The cytokine endothelin-1 (ET-1) is a kind of polypeptide composed of 21 amino acids which is produced and released by endothelial cells. When endothelial cells are damaged or their functions become maladjusted, the secretion of ET-1 will increase. Its molecular weight is estimated to be 2,492.0 Da [25]. ET-1 is not only one of the strongest vasoconstrictors but also is evaluated as a platelet aggregation promoting factor, having become the most popular target of various blood-activating and stasis-eliminating TCM [26-28].