As shown in Figure 3, the percentages of viable cells in 14 3 3epsilon concerning GFP and negative control GFP groups compared to that in blank group were 36. 6814. 09% and 71. 6812. 10%, respectively, which indi cated that the growth of Hep 2 cells decreased signifi cantly after 14 3 3epsilon GFP transfection. S phase arrest of Hep 2 cells with overexpression of 14 3 3epsilon Compared with the blank control and nega tive control GFP groups, a significant accu mulation of cells in the S phase of the cell cycle and a concomitant increase in the apoptotic sub G1 population were noted in the 14 3 3epsilon GFP group. In the control, negative and 14 3 3epsilon GFP groups, the proportions of S phase cells were 22. 473. 36%, 28. 173. 97% and 46. 156. 82%, respectively and the apoptotic sub G1 population significantly increased from 1.

231. 02% and 2. 921. 59% in the con trol and negative groups, respectively, to 13. 723. 89% in the 14 3 3epsilon GFP group. Inhibitors,Modulators,Libraries Increased apoptosis of Hep 2 cells transfected with 14 3 3epsilon Compared with blank control and negative control GFP groups, the number of the late apoptotic cells significantly increased in 14 3 3epsilon GFP group cells. The percentages of the apoptotic cells in control, negative and 14 3 3epsilon GFP groups were 0. 840. 25%, 1. 080. 24% and 2. 930. 13%, respectively, which showed significant differences among the different groups. Decreased invasiveness in Hep 2 cells transfected with 14 3 3epsilon Compared to those in the blank control and negative control GFP groups, the number of cells migrating across the membranes in the 14 3 3epsilon GFP group decreased dramatically.

The numbers of cells penetrating the filter membrane in the control, negative and 14 3 3epsilon GFP groups were 20. 651. 94, 17. 631. 04 and 9. 10. 24, Inhibitors,Modulators,Libraries respectively, which showed significant differences among the different groups. Discussion 14 3 Inhibitors,Modulators,Libraries 3epsilon is a member Inhibitors,Modulators,Libraries of the 14 3 3 protein family comprising Inhibitors,Modulators,Libraries a series of highly conserved small acidic pro teins of about 29 33 kDa. truly 14 3 3 proteins, which were originally identified as brain specific, are present in a wide range of organisms and tissues. These proteins nor mally exist as homo or heterodimers. The 14 3 3 dimer serves as an adaptor that couples with target proteins to compared with those in the clear surgical margin tissues. However, there was no significant correlation between mRNA and protein levels in LSCC, which could be caused by mechanisms such as inhibition of microRNAs in translation. There was also no relationship between 14 3 3epsilon expression levels and sex or age in patients suffering with LSCC, which shows that sex and age do not affect the expression levels of 14 3 3epsilon in LSCC.