(C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci 121: 2149-2156, 2011″
“Purpose: To evaluate in vivo sentinel lymph node (SLN) mapping by using photoacoustic and ultrasonographic (US) imaging with a modified clinical US imaging system.
Materials and Methods: Animal protocols were approved by the Animal Studies Committee. Methylene blue dye accumulation in axillary lymph nodes of seven healthy Sprague-Dawley rats was imaged by using a photoacoustic imaging system adapted from a clinical US imaging system. To investigate clinical translation, the imaging depth FG 4592 was extended up to 2.5 cm by adding chicken or turkey breast on top of the rat skin surface. Three-dimensional photoacoustic images were
acquired by mechanically
scanning buy Roscovitine the US transducer and light delivery fiber bundle along the elevational direction.
Results: Photoacoustic images of rat SLNs clearly help visualization of methylene blue accumulation, whereas coregistered photoacoustic/US images depict lymph node positions relative to surrounding anatomy. Twenty minutes following methylene blue injection, photoacoustic signals from SLN regions increased nearly 33-fold from baseline signals in preinjection images, and mean contrast between SLNs and background tissue was 76.0 +/- 23.7 (standard deviation). Methylene blue accumulation in SLNs was confirmed photoacoustically by using the optical absorption spectrum of the dye. Three-dimensional photoacoustic images demonstrate dynamic accumulation of methylene blue
in SLNs after traveling through lymph vessels.
Conclusion: In vivo photoacoustic and US mapping of SLNs was successfully demonstrated with a modified clinical US scanner. These results raise confidence that photoacoustic and US imaging can be used clinically for accurate, noninvasive imaging of SLNs for FG-4592 mouse axillary lymph node staging in breast cancer patients. (C) RSNA, 2010″
“This study investigated the potential use of mungbean (Vigna radiata (L.) Wilczek) protein hydrolysate (MPH) prepared from tryptic hydrolysis as an antioxidative hydrolysate and as a carrier for anticancer asiatic acid (AA). The antioxidant capacity of MPH was 0.67 and 0.46 mu mol Trolox equivalent (TE)/mg protein, as measured by oxygen radical absorbance capacity-fluorescein (ORAC(FL)) and Trolox equivalent antioxidant capacity (TEAC) assays, respectively. Freeze-drying in lactose excipient reduced the antioxidant capacity of MPH to 0.48 mu mol TE/mg protein in ORAC(FL) assay (P < 0.05) but,did not alter antioxidant capacity determined by TEAC assay (P >= 0.05). The genotoxicity of H(2)O(2) (50 mu M, 30 min) on hepatoblastoma HepG2 could be alleviated after HepG2 cells had taken up MPH after H(2)O(2) exposure (P < 0.05). Moreover, the inhibition concentration (IC(50)) of AA in HepG2 was lowered from 58.5 mu g mL(-1) of AA alone to 38.