melitensis 16M and 16MΔ vjbR with and without the addition of C 12 -HSL. Gene transcripts found to be altered by comparison of wild type and ΔvjbR, both with and without the Selleckchem C646 treatment of C12-HSL at an exponential and stationary growth phase. (DOCX 184 KB) Additional file 4: Table S4: Promoter(s) sequences and potential operons of downstream genes found to be altered by the deletion of vjbR and/or treatment of C 12 -HSL. Operons that are both found to be downstream of the predicted VjbR promoter
sequence and altered by comparison of wild type and ΔvjbR, both with and without the addition of C12-HSL at exponential or stationary growth phases. (DOCX 225 KB) Additional file 5: Table S5: Genetic loci identified with significant alterations in transcript levels between B. melitensis 16MΔ vjbR and 16MΔ vjbR
with the addition of C 12 -HSL. Altered gene transcripts uniquely identified by the treatment of C12-HSL to the B. melitensis 16MΔvjbR background. (DOCX 110 KB) References 1. Chaves-Olarte E, Guzman-Verri C, Meresse S, Desjardins M, Pizarro-Cerda J, Badilla J, Gorvel JP: Activation of Rho and Rab GTPases dissociates Brucella abortus internalization from Selleckchem AZD4547 intracellular trafficking. Cell Microbiol 2002,4(10):663–676.PubMedCrossRef 2. Gross A, Terraza A, Ouahrani-Bettache S, Liautard JP, Dornand J: In vitro Brucella suis Caspase inhibitor infection prevents the programmed cell death of human monocytic cells. Infect Immun 2000,68(1):342–351.PubMedCrossRef 3. Pizarro-Cerda J, Meresse S, Parton RG, van der Goot G, Sola-Landa A, Lopez-Goni I, Moreno E, Gorvel JP: Brucella abortus transits through the autophagic pathway and replicates
in the endoplasmic reticulum of nonprofessional phagocytes. Infect Immun 1998,66(12):5711–5724.PubMed http://www.selleck.co.jp/products/PD-0332991.html 4. Arellano-Reynoso B, Lapaque N, Salcedo S, Briones G, Ciocchini AE, Ugalde R, Moreno E, Moriyon I, Gorvel JP: Cyclic beta-1,2-glucan is a Brucella virulence factor required for intracellular survival. Nat Immunol 2005,6(6):618–625.PubMedCrossRef 5. Celli J, de Chastellier C, Franchini DM, Pizarro-Cerda J, Moreno E, Gorvel JP: Brucella evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum. J Exp Med 2003,198(4):545–556.PubMedCrossRef 6. Godfroid F, Taminiau B, Danese I, Denoel P, Tibor A, Weynants V, Cloeckaert A, Godfroid J, Letesson JJ: Identification of the perosamine synthetase gene of Brucella melitensis 16M and involvement of lipopolysaccharide O side chain in Brucella survival in mice and in macrophages. Infect Immun 1998,66(11):5485–5493.PubMed 7. Anand SK, Griffiths MW: Quorum sensing and expression of virulence in Escherichia coli O157:H7. Int J Food Microbiol 2003,85(1–2):1–9.PubMedCrossRef 8. Davies DG, Parsek MR, Pearson JP, Iglewski BH, Costerton JW, Greenberg EP: The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science 1998,280(5361):295–298.PubMedCrossRef 9.