Proc Natl Acad Sci USA 2008, 105:15499–15504.PubMedCrossRef 31. Rinke C, Schwientek P, Sczyrba A, Ivanova NN, Anderson IJ, Cheng PKA activator JF, click here Darling A, Malfatti S, Swan BK, Gies EA, et al.: Insights into the phylogeny and coding potential of microbial dark matter. Nature 2013,499(7459):431–437. doi: 10.1038/nature12352. Epub 2013 Jul 14PubMedCrossRef 32. Zong C, Lu S, Chapman AR, Xie XS: Genome-wide detection of single-nucleotide and copy-number variations of a single human cell. Science 2012, 338:1622–1626.PubMedCrossRef 33. Fitzsimons MS, Novotny M, Lo CC, Dichosa AE, Yee-Greenbaum

JL, Snook JP, Gu W, Chertkov O, Davenport KW, McMurry K, et al.: Nearly finished genomes produced using gel microdroplet culturing reveal substantial intraspecies genomic diversity within learn more the human microbiome. Genome Res 2013, 23:878–888.PubMedCrossRef 34. McLean JS, Lombardo MJ, Badger JH, Edlund A, Novotny M, Yee-Greenbaum J, Vyahhi N, Hall AP, Yang Y, Dupont CL, et al.: Candidate phylum TM6 genome recovered from a hospital sink biofilm provides genomic insights into this uncultivated phylum. Proc Natl Acad Sci USA 2013, 110:E2390-E2399.PubMedCrossRef 35. Kaur IP, Kuhad A, Garg A, Chopra K: Probiotics: delineation of prophylactic and therapeutic benefits. J Med Food 2009, 12:219–235.PubMedCrossRef 36. Sblattero D, Bradbury A:

Exploiting recombination in single bacteria to make large phage antibody libraries. Sclareol Nat Biotechnol 2000, 18:75–80.PubMedCrossRef 37. Ferrara F, Listwan P, Waldo GS, Bradbury ARM: Fluorescent labeling of antibody fragments using split GFP. PLoS One 2011,6(10):e25727. doi: 10.1371/journal.pone.0025727. Epub 2011 Oct 5PubMedCrossRef 38. Hanke T, Szawlowski P, Randall RE: Construction of solid

matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J Gen Virol 1992,73(Pt 3):653–660.PubMedCrossRef 39. Cabantous S, Terwilliger TC, Waldo GS: Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein. Nat Biotechnol 2005, 23:102–107.PubMedCrossRef 40. Claesson MJ, Sinderen DV, O’Toole PW: Lactobacillus phylogenomics, Äì towards a reclassification of the genus. Int J Syst Evol Microbiol 2008, 58:2945–2954.PubMedCrossRef 41. Messner P, Steiner K, Zarschler K, Schaffer C: S-layer nanoglycobiology of bacteria. Carbohydr Res 2008, 343:1934–1951.PubMedCrossRef 42. Sara M, Sleytr UB: S-Layer Proteins. J Bacteriol 2000, 182:859–868.PubMedCrossRef 43. Woyke T, Tighe D, Mavromatis K, Clum A, Copeland A, Schackwitz W, Lapidus A, Wu D, McCutcheon JP, McDonald BR, et al.: One bacterial cell, one complete genome. PLoS One 2010, 5:e10314.PubMedCrossRef 44. Woyke T, Sczyrba A, Lee J, Rinke C, Tighe D, Clingenpeel S, Malmstrom R, Stepanauskas R, Cheng JF: Decontamination of MDA reagents for single cell whole genome amplification. PLoS One 2011, 6:e26161.PubMedCrossRef 45.