In the course of PSNT, a different unfamiliar rat was placed inside the plastic cage on the opposite lateral chamber that had been empty through the Sociability session. The experimental rat was allowed to move freely and contact the plastic cages housing the strangers for ten minutes. Inter session intervals lasted 3 mi nutes. Rats behavior was recorded by a video camera mounted on the ceiling. The resulting video signal was relayed to a monitor and to an EthoVision image analyzer. The parameters analyzed in each and every lateral chamber had been frequency of entries, total duration and total distance traveled, Context and tone fear conditioning The apparatus consisted of a 21 21 49 cm situation ing chamber, Chamber walls have been made of gray Plexiglas, plus the ceiling was created of transparent Plexiglas to allow video recording. The grid floor was connected to a shock generator scrambler, The FC test encompassed three sessions.
education, context and tone. During instruction session, following a 120 seconds PF-4708671 concentration of acclimation towards the conditioning appar atus, three trials consisting from the 30 second tone exposure had been carried out. The last 2 seconds of each tone had been paired using a 1 mA foot shock. Tone and shock free of charge 60 second inter trial intervals have been used. Just after 24 hours, rats were placed for 240 seconds inside the coaching chamber, After 4 hours, the rats had been submitted to a tone test inside a white Plexiglas walls. Soon after 120 seconds of acclimation, a 120 second tone identical to that implemented in the coaching session was sounded without having any shock, In the course of education, context and tone tests, the rats be havior was recorded by a video camera mounted around the ceiling. The resulting video signal was relayed to a moni tor and to an EthoVision image analyzer.
Furthermore, 22 kHz USVs had been recorded, Behavioral parameters taken into account were frequency and duration of freezing and number of defecations. USVs had been collected by means of an ultrasound microphone placed by means of a hole in the middle of the test chamber roof approximately 21 cm above the shock floor. Shikimate The microphone was sensitive to 15 to 180 kHz frequencies with a flat frequency response be tween 25 and 140 kHz. It was connected by an Ultra SoundGate USB audio device to a personal laptop or computer, which recorded information at 250,000 Hz in 16 bit format and stored as. wav files for subsequent analysis. Sound files had been transferred to SASLab Pro for sonographic evaluation in addition to a quick Fourier transform was performed, USV parameters taken into account were number of calls emitted, peak amplitude and frequency, frequency modulation and get in touch with duration. Histological analyses At the end of behavioral testing, the animals had been deeply anesthetized and transcardially perfused with sa line, followed by 4% paraformaldehyde and 0.