013). TPMT activity seemed not to be affected. 6-Thioguanine nucleotide concentrations increased from 138 (IQR 119-188) to 235 (223-304) and to 265 pmol/8 x 10(boolean AND)8 (IQR 188-344), whereas 6-methyl mercaptopurine ribonucleotides concentrations decreased from 13230 (IQR 7130-17420) to 690 (IQR 378-1325) and 540 (IQR 240-790) pmol/8 x 10(boolean AND)8 at 4 and 12 weeks of combination therapy (both p<0.001).
Conclusion: Allopurinol
and thiopurine GW4869 combination-therapy seems to increase HGPRT and decrease XO activity in IBD patients, which at least in part may explain the observed changes in thiopurine metabolite concentrations. (C) 2013 European Crohn’s and Colitis Organisation. Published by Elsevier B.V. All rights reserved.”
“To investigate whether topical application of hesperin affords protection to Balb/C mice epidermis from UVB-induced cyclobutane pyrimidine dimers (CPDs).
A DNA damage model of UVB irradiation-induced mice epidermis selleck was established. The immunohistochemical staining and southwestern dot blotting were used for CPDs detection; western blotting was used for P53 detection.
Topical application of hesperidin on Balb/C mice skin significantly decreased the amount of epidermal CPDs 24 and 48 h after 180 mJ/cm(2) of UVB irradiation as compared to untreated mice. UVB-induced p53
expression was more pronounced in hesperidin-treated mice epidermis compared to that of untreated mice.
Taken RG-7112 in vitro together, these results suggest that topical hesperidin application promotes DNA photo-damage repair. Hesperidin is therefore a promising protective substance against UVB radiation.”
“BACKGROUND:
Limited data
are available on the predictors of atrial fibrillation (AF) recurrence in patients with chronic AF.OBJECTIVES:
To evaluate potential clinical, echocardiographic and electrophysiological predictors of AF recurrence after internal cardioversion for long-lasting AF.METHODS:
A total of 99 consecutive patients (63 men and 36 women, mean age 63.33 +/- 9.27 years) with long-standing AF (52.42 +/- 72.02 months) underwent internal cardioversion with a catheter that consisted of two defibrillating coils. Shocks were delivered according to a step-up protocol. Clinical follow-up and electrocardiographic recordings were performed on a monthly basis for a 12-month period or whenever patients experienced symptoms suggestive of recurrent AF.RESULTS:
Ninety-three patients (93.94 %) underwent a successful uncomplicated cardioversion, with a mean atrial defibrillation threshold of 10.69 +/- 6.76 J. Immediate reinitiation of AF was observed in 15 patients (15.78 %) of whom a repeated cardioversion restored sinus rhythm in 13 cases. Early recurrence of AF (within one week) was observed in 12 of 93 patients (12.90 %). At the end of the 12-month follow-up period, during which seven patients were lost, 42 of the 86 remaining patients (48.84 %) were still in sinus rhythm. Multivariate regression analysis showed that left atrial diameter (OR 1.