11). This difference was statistically significant. In addition, the areas of the NeuN- and Olig2-positive nuclei exhibited some notable overlap. All six of the cases studied were 1p loss-negative (figures not shown). Previous studies have shown that small numbers of OLCs exhibit Histone Methyltransferase inhibitor neuronal differentiation.[15] However, the exact morphological differences between OLCs and neurocytes remain controversial. OLCs exhibit non-specific ultrastructural features and round, heterochromatic nuclei. Intracytoplasmic organelles

are poor. Microtubules but not intermediate filaments are seen.[15] Oligodendrogliomas with the chromosome 1p/19q codeletion exhibit identical features.[16] The nucleus is heterochromatic and the cytoplasm contains mitochondria, a small rough endoplasmic reticulum (ER) and ribosomes, as well as a few microtubules. The neurocytes contain a small rough ER and are rich in mitochondria; however, direct synaptic attachments on the cell surface are rarely seen. In general, ganglion cells are regarded as being part of the tumor when they exhibit atypia. Daumas-Duport listed two reasons why floating neurons that lack atypia are not entrapped pre-existing neurons.[8] First,

no cytological RXDX-106 solubility dmso variations are seen within normal cortical neurons. Second, these neurons are always present in the subcortical white matter. Since the nuclear size generally correlates with the cytoplasmic size, our morphometric study indicated that the neurons in the specific glioneuronal element possessed cytological variations that are also seen in normal cortical neuron and that they were same in size but rounder compared to normal neurons. In addition, floating neurons were absent or extremely those rare in DNT lesions involving

the subcortical white matter in our study. Moreover, Miyanaga reported a case of DNT that extended into the subarachnoid space.[17] In that case, no floating neurons were identified in the specific glioneuronal element within the subarachnoid space. These observations strongly suggest that Daumas-Duport’s theory might indeed not be a valid assumption. Based on the above results, particularly the fact that Olig2 and NeuN are mutually exclusive, we naturally came to the conclusion that the NeuN-positive small and large cells observed within the element are in fact entrapped granular and pyramidal cells within the cortex. We also concluded that OLCs are essentially glial and not neuronal in nature. If our assumption is correct, then DNT might very well be pure glial tumors as opposed to glioneuronal tumors. Although OLCs lack both 1p/19 loss[18] and PDGFRα overexpression[19] which are characteristic features in oligodendrogliomas, OLCs otherwise share a common phenotype with oligodendrogliomas. In conclusion, our results suggest that DNTs are more akin to oligodendroglioma than glioneuronal tumors, although their biological and genetic nature is clearly distinguishing form oligodendroglioma.