21101053) for financial support and the scientific research project funds support of Hefei Normal University (2014cxy23). This work is also supported by the Anhui Provincial Science Research Projects (KJ2011Z301,KJ2012Z331). Natural Science Foundation of Anhui Province Science Research Projects (1308085 MB23, 1408085 MB30). References 1. Katsu Y, Kubokawa K, Urushitani H, Iguchi
T: Estrogen-dependent transactivation of amphioxus steroid hormone receptor via both estrogen and androgen response elements. Endocrinology 2010,151(2):639–648.CrossRef 2. Kozlowska-Tylingo K, Namiesnik J, Gorecki T: Determination of estrogenic endocrine disruptors in environmental samples-a review of chromatographic methods. Crit Rev Anal Chem 2010,40(3):194–201.CrossRef 3. Regal P, Nebot C, Vazquez BI, Cepeda A, Fente C: Determination of naturally occurring progestogens in selleck bovine milk as their oxime derivatives using high performance liquid chromatography-electrospray ionization-tandem mass spectrometry. J Sci Food Agric 2010,90(10):1621–1627.CrossRef 4. Wang L, Yang P, Li YX, Zhu CQ: A flow-injection chemiluminescence method for the determination
of some estrogens by enhancement of luminol-hydrogen peroxide-tetrasulfonated manganese phthalocyanine reaction. Talanta 2006,70(1):219–224.CrossRef 5. Jobling S, Nolan M, Tyler CR, Brighty learn more G, Sumpter Selleck Gefitinib JP: Widespread sexual disruption in wild fish. Environ Sci Technol 1998,32(17):2498–2506.CrossRef 6. Zhou LQ, Yang B, Xu YR, Yang GY, Hu QF: Determination of phenolic environmental estrogens in eggs by high performance liquid chromatography and sample preparation with matrix solid phase dispersion. Asian J Chem 2010,22(2):1141–1145. 7. Xu Q, Wu SY, Wang M, Yin XY, Wen ZY, Ge WN, Gu ZZ: Electrospun
nylon6 nanofibrous membrane as SPE adsorbent for the enrichment and determination of three estrogens in environmental water samples. Chromatographia 2010,71(5–6):487–492.CrossRef 8. Wang QL, Zhang AZ, Pan X, Chen LR: Simultaneous determination of sex hormones in egg products by ZnCl 2 depositing lipid, solid-phase extraction and ultra performance liquid chromatography/electrospray ionization tandem mass spectrometry. Anal Chim Acta 2010,678(1):108–116.CrossRef 9. Piwowarska J, Radowicki S, Pachecka J: Simultaneous determination of eight estrogens and their metabolites in serum using liquid chromatography with electrochemical detection. Talanta 2010,81(1–2):275–280.CrossRef 10. Mendez ASL, Deconto L, Garcia CV: UV derivative spectrophotometric method for determination of estradiol valerate in tablets. Quim Nova 2010,33(4):981–983.CrossRef 11. Liu ZH, Hashimoto T, Okumura Y, Kanjo Y, Mizutani S: Simultaneous analysis of natural free estrogens and their conjugates in wastewater by GC-MS. Clean-Soil Air Water 2010,38(2):181–188.CrossRef 12.