4 ± 0.2 hours. The training load was determined for each training mode (i.e.; resistance training and specific training). The resistance training load was determined according to previous criteria by multiplying the RPE score which was reported 30 minutes after the end of the training session using the modified 10-point

Borg scale – CR-10: RPE (session RPE) by the training volume (i.e., number of sets X number of repetitions) [17]. The training load of Selleck Lumacaftor the specific training was also assessed according to previous criteria by multiplying the session RPE by the training volume (i.e.; duration, in minutes, of the training session) [18]. Total training load, hereafter called training load, was measured as the summation (in arbitrary units) of the specific training loads and the resistance training loads

per week according to previously described criteria [19]. Training load, as determined by RPE method [19], was progressively increased throughout the training period as depicted in Figure 1. Figure 1 Illustration of the training load (as determined by the RPE method [19] ) progression throughout the intervention period. Jumping test CMJ performance assessment protocol consisted of 8 jumps with 60-second intervals between each attempt [20, 21]. The average of the 8 jumps was considered for MG132 analysis. CMJ was initiated from a standing position. Subjects were instructed to maintain their hands on their chest and freely determine the amplitude of the countermovement in order to avoid changes in jumping coordination [22]. Subjects were encouraged to jump as high as possible. Previous reports support the use of jumping

to measure the effects of creatine on lower limb performance [10, 23–25]. A strain-gauge force plate (AMTI BP600900; Watertown, EUA) was used to measure jumping performance. Data referring to the vertical ground reaction force component (Fy) were collected at a 1000 Hz. A Butterworth low pass (90 Hz cut off frequency) on-line filtering was also performed. Jumping height was determined by the impulse. The jumping performance was calculated by the following equation: where h is the height of jump, v is the vertical takeoff velocity, and g is the acceleration due to gravity. The data were analysed through the MatLab R2009b software (Mathworks, EUA). Dietary intake Dietary O-methylated flavonoid intake was assessed by means of 3, 24-hour dietary recalls undertaken on separate days (2 week days and 1 weekend day) using a visual aid photo album of real foods. Energy, macronutrient and creatine intake were analyzed by the software Virtual Nutri (Sao Paulo, Brazil). Supplementary creatine was not considered in the analysis. Creatine supplementation protocol and blinding procedure The subjects from the creatine group received 20 g/d of creatine monohydrate (Probiótica, Sao Paulo, Brazil) for 1 week divided into 4 equal doses, followed by single daily doses of 5 g for the next 6 weeks.