[41] A simple HPTLC method has been developed for the simultaneous determination of isoorientin, isovitexin, PXD101 orientin, and vitexin, both pure and in commercial samples of bamboo-leaf flavonoids. It was found that HPTLC is a simple, precise, specific, and accurate and can be used for manufacturing QC of bamboo-leaf flavonoids or for governmental regulatory purposes.[42] Many such reports[43�C49] present the evidence of utilization of HPLTC in fingerprinting analysis of drugs of natural origin, and hence, the increasing acceptance of natural products is well suited to provide the core scaffolds for future drugs; there will be further developments in the use of novel analytical techniques in natural products drug discovery campaigns.

HPTLC IN OTHER FIELDS In recent years, HPTLC is a globally accepted practical solution to characterize small molecules in quality assessment throughout the developing world. HPTLC is used for purity control of chemicals, pesticides, steroids, and water analysis.[50] HPTLC is also widely used for analysis of vitamins, water-soluble food dyes, pesticides in fruits, vegetables, and other food stuffs.[51] Beate et al[52] reported the analysis of stem cell lipids by offline HPTLC-MALDI-TOF MS. HPTLC is useful in detecting chemicals of forensic concern,[53,54] including abuse drugs, poisons, adulterations, chemical weapons, and illicit drugs. CONCLUSIONS Applications of HPTLC for phytochemical analysis, biomedical analysis, herbal drug quantification, analytical analysis, finger print analysis, and HPTLC future to combinatorial approach, HPTLC-MS, HPTLC-FTIR and HPTLC-Scanning Diode Laser made HPTLC a power analytical tool in the field of analysis.

It is noteworthy that utilization of instrumental HPTLC toward the analysis of drug formulations, Bulk drugs, natural products, clinical samples food stuffs, environmental, and other relevant samples will increase in the future. Footnotes Source of Support: Nil Conflict of Interest: None declared.
Extraction of 1 kg powder of the entire plant was carried out in a round-bottom flask at a temperature <50��C using petroleum ether (Spectrochem Pvt. Ltd., Mumbai, India). The present study was carried out after the pharmacological activity (anti-ulcer, diuretic and laxative) using various extracts of O. esculentum suggested that petroleum ether extract had the best pharmacological action.

So, petroleum ether extract was further investigated for its phytochemical constitution (thereby following bioactivity-guided fractionation). The dried petroleum ether extract was further fractionated between n-hexane and water, carbon tetrachloride and water, toluene and water, diethyl ether and water, dichloromethane and water, GSK-3 n-butanol and water, chloroform and water, ethyl acetate and water in this order and finally between 80% methanol and water.