Animals

Animals www.selleckchem.com/products/azd9291.html Healthy male Wistar strain albino rats, weighting between 140 and 160 g were used for this study. Rats were housed in our well ventilated animal house having six animals in polypropylene made cages and maintained standard condition with controlled temperature (25 �� 3��C), relative humidity (45 �� 5��C) and 12:12 h dark:light cycle. The animals were fed with standard laboratory diet and allowed to drinking water ad libitum. Experiments were conducted following the guidelines of our Institutional Animal Ethics Committee (IAEC). Chemicals STZ was purchased from Sigma, USA. Other chemicals like adenosine tri phosphate (ATP), nucleotide adenine dinucleotide phosphate (NADP), 2-[4-(2-Hydroxyethyl) 1-piperazinyl] ethanesulfonic acid (HEPES), were purchased from Sigma-Aldrich Diagnostic Ltd.

or Sisco Research Laboratory (SRL), India. Kits for the assessment of serum lipid profile level and glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) activities were purchased from Span Diagnostic Ltd. Surat, India. Induction of diabetes Rats were made diabetic by a single intramuscular injection of streptozotocin (STZ) at the dose of 40 mg/kg body weight in 0.1 M citrate buffer (pH 4.5).[3,14] Diabetic state was confirmed on seventh day of STZ injection considering the measurement of FBG as relevant biomarker. Animals with FBG level more than 300 mg/dL were selected for this experiment. Homeopathic remedy The homeopathic remedy S jambolanum (��), was purchased from ��Hahnemann Publishing Company (Hapco)��, 165, Bepin Behari Ganguly Street, Kolkata, India.

From this 1 ml of mother tincture of S jambolanum was finally diluted with 20 ml of double distilled water to make the stock solution. Each rat were fed 1 drop (0.06 ml) of S jambolanum twice a day from the stock solution using gavage. The drug feeding was made before food delivery in subsequent days till they were sacrificed for analysis. Experimental design To fulfil the aims of this study, animals were divided into four groups and each groups comprising of 6 rats. Group wise animal distribution was as follows: Untreated control Animals of this group were treated with 0.6 ml of diluted ethanol (vehicle) for 40 days at the time of mother tincture treatment to diabetic animals. Diabetic control Animals of this group were given vehicle solution for 40 days at the same time.

Diabetic + S jambolanum Animals of this group were treated Dacomitinib with 0.06 ml of mother tincture of S jambolanum for 40 days. The duration of the experiment was 40 days. On forty-first day, all the animals were sacrificed by decapitation. Blood was collected from dorsal aorta by a syringe, the serum was separated by centrifugation at 3000 g for 10 mins for the measurements of serum lipid profile level and activities of GOT and GPT in serum.

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