Based on present literature, we hypothesized to find a loss in body mass as has previously reported for ultra-cycling [21, 24, 36] and non-stop ultra-endurance races [15, 22, 24, 26]. We hypothesized

that this type of MTB races would lead to an increase in foot volume due to peripheral oedema. Methods Participants The present work combines data from two 24-hour races held in the Czech Republic in 2012. Subjects were recruited via pre-race emails and during race registration. A total of 28 (22 men and 6 women) recreational 24-hour ultra-MTBers in the solo category from the ‘Czech Championship 24-hour MTB 2012’ in Jihlava city in the Czech Republic and 24 (18 men and 6 women) Pictilisib solubility dmso ultra-MTBers from the ‛Bike Race Marathon MTB Rohozec 24 hours’ in Liberec city in the Czech Republic in the solo category consented to participate in the study. Of those, 37 men and 12 women finished the race successfully. One cyclist had to give up due to technical problems and two athletes because of medical complications. Athletes were informed that participation was Aurora Kinase inhibitor voluntary and that the project had received approval in accordance with the law (No. 96/2001 Coll. M. S. on

Human Rights and Biomedicine and Act No. 101/2000 Coll. Privacy). The pre-race anthropometry and training data of the participants are presented in Table  1. Table www.selleckchem.com/products/ly2874455.html 1 The pre-race experience and training parameters (n = 49)   Male ultra-MTBers Female ultra-MTBers (n = 37) (n = 12) M ± SD M ± SD Years as active biker (yr) 9.2 ± 5.8 8.8 ± 5.9 Number of finished ultra-marathons (n) 8.0 ± 6.5 6.7 ± 5.3 Personal best km in 24 hour (km) 315.5 ± 89.7 279.6 ± 106.7 Total hours weekly (h) 10.5 ± 5.3 10.2 ± 5.5 Weekly cycling kilometers (km) 225.8 ± 149.5 191.8 ± 134.5 Weekly cycling hours (h) 9.9 ± 5.1 9.2 ± 5.2 Mean cycling intensity (beat/min) 133.8** ± 7.6 134.5** ± 22.8 Mean cycling speed (km/h) 23.0** ± 3.6 21.1** ± 5.3 Longest trail (km) 176.8** ± 84.7 141.7** ± 75.5

Amount of km in 2011 (km) 7,107.5 ± 5,782.4 5,696.9 ± 5,037.9 Results are presented as mean ± SD; * = P < 0.05, ** = P < 0.001. Races details The first measurement was performed at the 3rd edition of the ‘Czech Championship 24-hour MTB 2012’ in Jihlava. The ultra-MTBers began the race at Methamphetamine 12:00 on 19th May 2012 and finished at 12:00 on 20th May 2012. The course comprised a 9.5 km single-track with an elevation of 220 m. A single aid station, located at the start/finish area was provided by the organizer where a variety of food and beverages such as hypotonic sports drinks, tea, soup, caffeinated drinks, water, fruit, vegetables, energy bars, bread, soup, sausages, cheese, bread, chocolate and biscuits were available. The ultra-MTBers could also use their own supplies in their pit stops. Temperature was +16˚C at the start, rose to a maximum of +20˚C, dropped to +6˚C during the night and rose to +23˚C from the morning of the next day till the end of the race.