(C) 2011 American Institute of Physics. [doi:10.1063/1.3627180]“
“We describe a technique for combined fluid-gas exchange and phacoemulsification 2 weeks after macular hole surgery to achieve rapid visual rehabilitation. The procedure uses a standard cataract set and requires minimal vitreoretinal expertise. We encountered no significant complications

and obtained long-term outcomes comparable to those with conventional management.”
“Asiaticoside AZD0530 price has been identified as the most active compound in Centella asiatica. In order to screen a large number of plant samples for the presence of asiaticoside, a rapid and simple technique is required that utilizes small quantities for test samples. In this study, an immunochromatographic strip test has been developed for the detection of asiaticoside in plant samples that uses a monoclonal antibody against asiaticoside. The limit of detection for the strip test was 12.5 mu g/ml. Immunoassay using monoclonal antibodies could be useful for the determination of small quantities of asiaticoside selleck screening library in plant extracts.”
“Purpose: The purpose of this study was to test the effect of the combination of mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) incorporated into a fluorohydroxyapatite

(FHA) scaffold on bone regeneration in cylindrical defects in the edentulous mandibular ridge of minipigs.

Materials and Methods: Two mandibular premolar teeth were extracted bilaterally in 8 adult minipigs. After 2 months, 4 Standardized defects of 3.5 mm diameter and 8 mm depth were created in each root site. The defects were randomly grafted with autogenous mandibular bone, FHA alone, PRP-FHA, or MSCs-PRP-FHA. A resorbable collagen membrane was placed over the defect area

and the flaps were sutured. The animals were sacrificed 3 months later and biopsy samples were taken from the defect sites for histologic and histomorphometric assessment.

Results: There was no evidence of inflammation or adverse tissue reaction with either treatment. MSCs-PRP-FHA-treated sites showed new vital bone between residual grafting particles. PRP-FHA- and FHA-treated LY3039478 chemical structure sites showed residual particles in a background of marrow soft tissue with a moderate quantity of newly formed bone. Autogenous bone (46.97%) and MSCs-PRP-FHA (45.28%) produced a significantly higher amount of vital bone than PPP-FHA (37.95%), or FHA alone (36.03%). Further, the MSCs-PRP-FHA-treated defects showed a significantly higher percentage of contact between graft particles and newly formed bone compared with PRP-FHA and FHA group (59.23% vs 48.37% and 46.43%, respectively).

Conclusions: Our results Suggest that, in this animal model, the addition of MSCs to PRP-FHA enhances bone formation after 3 months.