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Time-Domain Method. Boston: Artech House; 1995. Competing interests The authors declare that they have no competing interests. Authors’ contributions MYuT, BNK, VAK, and PST searched for the sample processing regimens, SEM, TEM, AFM, spectroscopic, and SERS measurements. MIS provided the opal-like substrates. VNB coordinated the project as a whole. MYuT provided a preliminary version of the manuscript. NGK analyzed all data, wrote the final version of the manuscript, and arranged all figures. All authors read and approved the final manuscript.”
“Background Silver nanostructures have

attracted much attention due to unique electrical, optical, and biocompatible properties that are applicable to chemical sensors, catalysts, interconnects in micro or nano devices, plasmonics, and photonics [1–5]. The chemical properties of Ag nanostructures are determined by their morphology, size, crystallographic plane, and alloying composition [6–8]. Among various silver nanostructures, nanoplates or nanosheets, particularly, have been intensively investigated because they have the size- and shape-sensitive surface plasmon resonance bands [1, 8–12]. Until now, two-dimensional 4-Aminobutyrate aminotransferase silver nanostructures have been fabricated using surfactants (capping agent) [6, 13], sacrificial materials [14], and hard templates (porous alumina) [15]. Although these methods have the merits of controlling the morphology and size of Ag nanostructures, they are complicated and costly. A chemical route without any surfactants led to the large-scale synthesis of micrometer-sized Ag nanosheets (approximately 15 μm in size and 28 nm in thickness) after the addition of a small quantity of H2PdCl4 as seeds for the growth of Ag nanosheets [16]. With such solution-based methods, colloidal nanosheets were randomly dispersed in a liquid before being used for their purposes.

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