Table 1 also reveals that the overall susceptibility rates of ciprofloxacin, doxycycline, and

sparfloxacin against all the selleck compound isolates were 97%, 92%, and 98% at pH 5.0; and 98%, 94%, and 99% at pH 7.0, respectively. Fifty-one isolates were resistant to rifampicin at both pH conditions (particularly the isolates from the Northern (n=28) and Coastal (n=18) regions), whereas Inhibitors,research,lifescience,medical 39 and 27 isolates were resistant to tetracycline at pH 5.0 and pH 7.0, respectively. No significant differences were observed regarding each individual antibiotic between pH 5.0 and pH 7.0, with the exception of the effect of tetracycline against the Southern region isolates, where the susceptibility was decreased at pH 5.0 compared with that at pH 7.0 (17 vs. 27 isolates; P<0.0007). Finally, 100% of the isolates were resistant to streptomycin. Table 1 Inhibitors,research,lifescience,medical Effect of medium pH levels on MICrange and MIC90 and the susceptibility percentage (Susc.%) of some antibiotics against B. melitensis isolates collected from different Syrian regions Figures 1 and ​and22 present the data on the effects of the antibiotic combinations at pH 7.0 and pH 5.0, respectively, on 24 selected Brucella isolates. The rifampicin-doxycycline combination showed a synergistic activity against 19 and 17 isolates at pH 7.0 and pH 5.0, respectively. Inhibitors,research,lifescience,medical The ciprofloxacin-doxycycline,

ciprofloxacin-sparfloxacin, and rifampicin-sparfloxacin combinations were indifferent against 20, 22, and 17 isolates at pH 7.0, respectively; and against 20, 22, and 13 isolates at pH 5.0, respectively. The rifampicin-tetracycline and rifampicin-streptomycin Inhibitors,research,lifescience,medical combinations showed additive activities against 12 and 7 isolates at pH 7.0; and against 12 and 5 isolates at pH 5.0, respectively. However, the ciprofloxacin-streptomycin and ciprofloxacin-tetracycline combinations demonstrated antagonistic activity against 9 and 6 Brucella isolates at pH 7.0; and against 13 and 9 isolates at pH

5.0, respectively. Figure 1 This is a representation of the activity of the antibiotic combinations at pH 7.0. R: Rifampicin; T: Tetracycline; D: Doxycycline; C: Ciprofloxacin; Inhibitors,research,lifescience,medical S: Sparfloxacin; ST: Streptomycin; Anta: Antagonism; Ind: Indifference; Add: Additive; Syn: Synergy Figure 2 This figures illustrates the activity of the antibiotic combinations at pH 5.0. R: Rifampicin; T: Tetracycline; D: Doxycycline; C: Ciprofloxacin; S: Sparfloxacin; ST: Streptomycin; Anta: Antagonism; Ind: Indifference; Add: Additive; Syn: Adenylyl cyclase Synergy Discussion Brucella spp. infect macrophages replicating within the phagolysosomes at a pH of 5.0.16 Theoretically, antibiotics that are able to penetrate the phagolysosomal compartment and function under acidic conditions could be used as monotherapy for the treatment of Brucella. However, in practice, neither doxycycline nor rifampicin (both of which meet these criteria) is effective as a monotherapeutic agent.1,14 Garcia-Rodriguez et al.