Taking together the structure of the SUMO 1 modified TDG CAT protein and our NMR data, somehow the SUMO 1 con jugation rather acts on the TDG C terminal conformation with no or little impact on Inhibitors,Modulators,Libraries the TDG RD conformation. In contrast, the SUMO 1 non covalent binding to the C terminal SBM is able to structurally modify both the N and C terminal regions of TDG and sumoylated TDG. Based on the observations reported here, we conclude that SUMO 1 does not adopt the same orientation as in the sumoylated protein. Interestingly, SUMO 1 non covalent binding leads to a partial RD displacement from its CAT interface indicating an effect of steric hindrance rather than overlapping binding interfaces on the CAT domain which is in good agreement with our previous suggestion for the putative localization of the RD interface on the CAT domain.
SUMO 1 does not interact with the C terminal SBM in presence of DNA It has been shown that SUMO 1 intermolecular binding is strongly reduced by TDGs association with DNA. Given our previous results concerning TDG RD DNA interactions, we have examined the effect of DNA heteroduplexes containing a G,U or a G,T mismatch on TDG conformation in the presence of SUMO 1. Some weak additional Inhibitors,Modulators,Libraries resonances matching with those of the isolated TDG N terminus bound to DNA heteroduplexes are observed on the 15N labeled TDG HSQC spectrum suggesting that DNA substrates containing either a normal G,C pair or a G,T U mismatch can displace similarly TDG RD from its TDG CAT interacting surface. Furthermore, no signal perturbation of TDG RD or A328 A345 region was observed upon SUMO 1 addition.
These data indicate that a DNA heteroduplex containing either a G,U or a G,T mismatch induces a conformational Entinostat modification of TDG RD, this effect being independent of SUMO 1 being present or not, and prevents SUMO 1 binding to the C terminal SBM which is in accordance with pre vious works. DNA binding to TDG CAT likely modifies the SBM2 conformation or accessibility so that it prevents any SUMO 1 interactions. We can not exclude that SUMO 1 could modify the binding affinity of TDG to DNA as it has been shown previously in an indirect manner. However, given the dissociation constant of the TDG DNA complex and the relatively high protein concentrations that must be used for NMR studies, Inhibitors,Modulators,Libraries the SUMO induced decrease of TDG DNA affi nity is not strong enough to be detected since, with a 20 uM sample, TDG, and more particularly the RD, Inhibitors,Modulators,Libraries is still satu rated with DNA whether SUMO is present or not.
SUMO 1 stimulates the glycosylase activity of TDG and TDG E310Q Although intermolecular SUMO 1 binding did not occur in presence of DNA or with the C terminal SBM mutation, we have observed a stimulation of the Brefeldin A IC50 glyco sylase activity of wild type and E310Q mutant TDG pro teins.