Therefore, it was concluded that the use of CoxAbic® as a method of vaccination offers at least the same level of protection and economic advantage as those commonly accepted and used in the poultry market. Further evidence of the effectiveness of the maternal immunization approach in the field was obtained in Thailand and South Africa. In a challenge trial in Thailand, three groups of vaccinated birds – CoxAbic®, a commercial live vaccine and salinomycin treated ABT-263 datasheet – were challenged with 60 000 virulent E. tenella oocysts orally. Lesion scores between the three flock groups revealed that the CoxAbic® vaccinated groups had the lowest lesion score (<0·5) at 24, 30 and 35 days of age. In contrast, live

vaccine treated flocks had a lesion score >2 during the same period, whilst salinomycin treated Cilomilast mouse flocks peaked at 30 days of age with a score >2·5, but recovered to ∼1·0 at day 35 (72), again confirming the effectiveness of vaccination with CoxAbic®. These results demonstrated that maternal immunization with gametocyte antigens provides the potential for controlling coccidiosis under different rearing conditions in various climates and environmental surroundings. The basis of control, rather than eradication, means that both sexual and asexual stage protective immunity develops in the birds.

Importantly, several recent studies demonstrated the conserved and functional importance of the two gametocyte antigens, Gam56 and Gam82, and explained why their inclusion in the vaccine formula confers protection against a range of Eimeria species (76). Concurrent to development of CoxAbic®, studies were conducted to characterize the Gam56 and Gam82 antigens that are the main components of the vaccine. Initial studies showed that Gam56 and Gam82 are glycoproteins (77) and further immunofluorescence studies

localized these antigens to the wall-forming bodies of the macrogametocyte and in the oocyst wall (78). These two antigens were identified as key players in the formation of the oocyst wall (54,69,79,80). The oocyst wall, which facilitates the transmission of Eimeria by protecting Buspirone HCl the parasite when it is in the outside world, originates from the fusion of specialized organelles – wall-forming bodies (WFB’s) – found in the macrogametocytes of Eimeria (78). During maturation of the macrogametocyte, the WFB’s align beneath the cell surface before degranulating and releasing Gam56 and Gam82 (Figure 1b). The proteins, and/or truncated versions thereof, are then believed to cross-link via dityrosine bonds to form the resilient wall structure (81). The inclusion of these proteins in CoxAbic® means that the stimulated antibodies probably interfere with the formation of cross-link’s between the proteins (Figure 1b), and therefore, prevent effective transmission by interrupting oocyst wall formation (72,82).