0 with sodium phosphate buffer (pH 6.0) for the proteolytic sensibility assay. To evaluate the effect of NaCl concentration on the activity of rEntA, an overnight culture of L. ivanovii ATCC19119 was diluted to 105–6 CFU/ml in fresh MHB medium (3% FBS). Ten microliters of purified rEntA and 10 μl of NaCl solution were added to 80 μl of diluted cell culture. The final rEntA concentration was 4 × MIC, and the final NaCl concentrations
were 0, 25, 50, 100, 200, and 400 mM. Samples without rEntA were used as controls. selleck chemicals All samples were incubated at 37°C for 10 h. The CFU of tested strains was determined. All tests were performed in triplicate. Acknowledgments The authors wish to acknowledge Prof. Yang Fuquan, Ph.D., in the Proteomics Platform Laboratory, Institute of Biophysics, Chinese Academy of Sciences, for his coordination of the MALDI-TOF MS analysis. selleck kinase inhibitor In addition, all other experiments described in this paper were run in the Gene Engineering Laboratory, Feed Research Institute, Chinese Academy of Agricultural Sciences. This work was supported by the National Natural
Science Foundation of China (No. 31372346, No. 31302004 and No. 30972125), the Project of National Support Program for Science and Technology in China (No. 2013BAD10B02 and No. 2011BAD26B02), and the AMP Direction of Innovation Program of Agric Sci & Tech in CAAS (2013–2017). References 1. Lohans CT, Vederas JC: Development of
Class IIa bacteriocins as therapeutic agents. Int J Microbiol 2012, 2012:1–13.CrossRef 2. Cotter PD, Hill C, Ross RP: Bacteriocins: developing innate immunity for food. Nat Rev Microbiol 2005, 3:777–788.PubMedCrossRef 3. Ennahar S, Deschamps N: Anti- Listeria effect of enterocin A, produced by cheese-isolated Enterococcus faecium EFM01, relative to other bacteriocins from lactic acid bacteria. Axenfeld syndrome J Appl Microbiol 2000, 88:449–457.PubMedCrossRef 4. Cotter PD, Ross RP, Hill C: Bacteriocins-a viable alternative to antibiotics? Nat Rev Microbiol 2012, 11:95–105.PubMedCrossRef 5. Blay GL, Lacroix C, Zihler A, Fliss I: In vitro inhibition activity of nisin A, nisin Z, pediocin PA-1 and antibiotics against common intestinal bacterial. Lett Appl Microbiol 2007, 45:252–257.PubMedCrossRef 6. Engelbrecht F, Domínguez-Bernal G, Hess J, Dickneite C, Greiffenberg L, Lampidis R, Raffelsbauer D, Daniels JJ, Kreft J, Kaufmann SH: A novel PrfA-regulated chromosomal locus, which is specific for Listeria ivanovii , encodes two small, secreted internalins and contributes to virulence in mice. Mol Microbiol 1998, 30:405–417.PubMedCrossRef 7.