18), and the nrfA (SO3980) genes. cymA (SO4591; ratio 0.39), the prismane protein hcp gene (SO1363), and neighboring protein hcr gene (SO1364), both of which were strongly repressed (ratios ≤ 0.13) and have been associated with the nitrate reduction pathway [24–27], did not show evidence of EtrA binding sites. Also indirectly down-regulated were the fumarate reductase genes Cediranib mouse frdAB (SO0398-0399) and fccA (SO0970), the ackA and the pta (SO2915-16) genes involved

in acetate production and the ppc (SO0274) gene encoding an acetate phosphoenol pyruvate carboxylase. The hyaCBA (SO2097-2099) genes encoding a quinone-reactive Ni/Fe hydrogenase were highly indirectly repressed (ratio ≤ 0.11). Among the genes identified as directly down-regulated are all the genes in the operon that encodes the anaerobic DMSO reductase (dmsAB) HM781-36B chemical structure (SO1428-32), the cydAB

genes (SO3285-3286) encoding a cytochrome d oxidase complex, as well as genes involved in metabolism of organic compounds such as the pflAB (SO2912-2913). Other down-regulated genes grouped in different categories included genes encoding ABC transporters (cydCD [SO3779-3780], SO4446-4448), TonB-dependent receptors (nosA [SO0630]), and L-lactate permease (lldP [SO0827]) and a putative lactate permease (SO1522). The only gene directly down-regulated from this later group is lldP (SO0827), for which an EtrA binding site was predicted (Table 3). As expected, the cDNA for etrA, shows no significant hybridization signal in EtrA7-1 mutant (ratio 0.05). Stress response caused by the etrA deletion We detected induction of

genes from Carbohydrate various categories, which have been associated with stress response i.e., starvation, phage infection and oxidative stress, possibly due to accumulation of nitrogen oxide reactive species. Up-regulated genes (Additional file 1) were dominated by genes grouped in “”Other categories”". The majority of up-regulated genes were phage-related. For BYL719 in vitro example, 25 genes of the LambdaSo phage (SO2940-2974), a gene encoding a viral capsid protein of the MuSo1 phage (SO0675), and genes of MuSo2 phage (SO2684-2685, SO2687, SO2702) were up-regulated. In contrast, the gene encoding the LambdaSo phage transcriptional regulator of the Cro/CI family (SO2990) was down-regulated (ratio 0.43). Transcriptional changes of most of these genes are likely indirect effects due to the deletion of the etrA gene and only for the LambdaSo phage genes S02957-2962 was an EtrA binding site predicted. The category “”Transport and binding proteins”" contains a large number of genes associated with stress response.