In this study we examined the modulation of apoptosis pathway genes in islets exposed to hydrogen

peroxide, peroxynitrite, hypoxia, and cytokines. We observed parallel induction of pro- and antiapoptotic pathways and identified several novel genes including BFAR, CARD8, BNIP3, and CIDE-A. As BNIP3 is an inducer of autophagy, we examined this pathway in MIN6 cells, a mouse beta cell line and in human islets. Culture of MIN6 cells under low serum conditions increased the levels of several proteins in autophagy pathway, including ATG4, Beclin 1, LAMP-2, and UVRAG. Amino acid deprivation led to induction of autophagy in human islets. Preconditioning of islets with inducers of autophagy

protected them from hypoxia-induced apoptosis. However, induction of autophagy during hypoxia exacerbated apoptotic cell death. ER stress led to A-1210477 induction of autophagy and apoptosis in beta cells. Overexpression of MnSOD, an enzyme that scavenges free radicals, resulted in protection of MIN6 cells from cytokine-induced apoptosis. Ceramide, a mediator of cytokine-induced injury, reduced the active phosphorylated form of Akt and downregulated the promoter activity of the antiapoptotic gene bcl-2. Furthermore, cytokine-stimulated JNK pathway downregulated the bcl-2 promoter activity which was reversed by preincubation with SP600125, a JNK inhibitor. Our findings suggest that beta BI 6727 purchase cell apoptosis by multiple stresses CCI-779 mouse in islets isolated for transplantation is the result of orchestrated gene expression in apoptosis pathway.”
“Dynamic holographic recording under linear polarized writing beams at 632.8 nm was performed oil two different new spirooxazine doped PMMA matrices assisted by UV light. Thermal rate constants of the two holographic storage medium were determined. It was found that diffraction efficiency and erasure dynamics of the holographic gratings in the two polymer films; were dependent

oil the thermal stability and aggregation of spirooxazine molecules in PMMA matrices. During erasure of the holographic gratings, only, in the polymer film with a lower thermal rate constant, a nearly instantaneous increase of diffraction efficiency was observed, resulting from the formation of a transient orientation grating. A theoretical description of the transient orientation grating, competing with isomerization grating’s, agrees well with experimental results. The interaction between the chromophores and matrices was also discussed. (C) 2008 Wiley Periodicals, Inc. J Appl Polym Sci 111: 2157-2162, 2009″
“Many variables may influence success rates after intrauterine insemination (IUI), including sperm quality in the native and washed semen sample.