CNE1-LMP1 cells were treated with the small molecule selleck inhibitor WHI-P131, a specific inhibitor of STAT3 phosphorylation at residue tyrosine 705 and serine

727. Both the promoter activity (Figure  4C) and the protein level (Figure  4D) of cyclin D1 decreased greatly upon WHI-P131 treatment. Treatment Veliparib in vivo with PD98059, a chemical inhibitor that blocks the nuclear translocation of STAT3, also decreased cyclin D1 promoter activity (Figure  4C) and protein expression (Figure  4D). On the other hand, the data in Figure  4C and Figure  4D indicated that AG1478, an EGFR specific tyrosine kinase inhibitor, decreased the transcriptional activity of the cyclin D1 promoter and protein level. WHI-P131 was less efficient in the presence of PD98059 in cyclin D1 transcription (Figure  4C) but not cyclin D1 protein level

(Figure  4D). siSTAT3 or WHI-P131 induced a stronger inhibition of cyclin D1 promoter activity than siEGFR or AG1478. Taken together, these data FRAX597 concentration suggest that both EGFR and STAT3 signaling pathways are involved in the transcriptional activity of Cyclin D1 promoter and protein levels. LMP1 regulated the nuclear EGFR and STAT3 binding to the cyclin D1 promoter region directly Next, we addressed whether the nuclear interaction of EGFR and STAT3 associates with the cyclin D1 promoter directly using electrophoresis mobility shift assay (EMSA) in CNE1 and CNE1-LMP1 cells. The probes, which contain EGFR or STAT3 binding sites according to the previous report [31], were labeled with biotin. As shown in Figure  5A, we found significant binding of nuclear protein to cyclin D1 (lane 2) while LMP1 promoted more nuclear protein binding (lane 3), indicating that LMP1 promoted STAT3 binding to the cyclin D1 promoter. The complex in CNE1-LMP1 cells was abolished by adding cold STAT3 binding sequence (Figure  5A, lane 4) but not by a mutation in the STAT3 binding

sequence (Figure  5A, lane 5) or a nonspecific binding sequence (Figure  5A, lane 6). After we mutated the plasmid containing functional mutated cyclin D1 promoters, we Tyrosine-protein kinase BLK could not detect the band in either CNE1 or CNE1-LMP1 cells (lanes 8 and 9 of Figure  5A). After the CNE1 cells were treated with IL-6 to induce STAT3 activation, we observed STAT3 binding in the cyclin D1 promoter (Figure  5B). After the CNE1-LMP1 cells were treated with the STAT3 inhibitors WHI-P131 and PD98059 (Figure  5B), we observed that STAT3 binding in the cyclin D1 promoter decreased. Taken together, LMP1 promoted STAT3 binding to the Cyclin D1 promoter. Figure 5 LMP1 increased the binding ability of transcription factors EGFR and STAT3 to cyclin D1 promoter in vitro . (A) STAT3 binding activities within the cyclin D1 promoter were examined by EMSA.

On the other hand, the maximum nanohole depth is achieved at a longer annealing time for a lower As flux. Moreover, once the nanohole maximum depth has been achieved, AZD4547 cost a further annealing time under As flux leads to a reduction of the nanohole depth. Figure 5 Hole depth as a function of the annealing time of Ga droplets. Under two different arsenic fluxes (0.08 and 1.40 ML/s) at constant substrate temperature T

S = 500°C. In view of our results, we can outline the following processes running during the annealing of Ga droplets under As exposure, which are associated to the characteristic evolution rates: local etching by the metallic Ga droplets (I) active until the Ga droplets are consumed by GaAs growth (II) and evolution of nanoholes to shallower

structures (III). In this context, it can be explained that the annealing time for reaching the nanohole maximum depth Caspase inhibitor by nanodrilling beneath the Ga droplet (process I) mTOR inhibitor depends on As flux, as the consumption rate of the droplet by GaAs formation (process II) depends on As flux in MBE growth under growth conditions limited by V element [26]. Once the etching is over by consumption of the Ga droplets (nanohole maximum depth achieved), a further annealing time under As flux leads to a reduction of the nanohole depth due to the incorporation of Ga atoms at B-type walls coming from the lateral movement of Ga surface atoms during the annealing process, a behavior observed in any patterned surface at high temperature [36]. Conclusions In this work, we have studied the formation of nanoholes on GaAs(001) substrates produced after Ga droplet epitaxy at T S = 500°C. Our results show that nanodrilling CHIR-99021 order of the GaAs(001) substrate is only possible

in the presence of arsenic. We have identified three processes that take place when Ga droplets are exposed to an arsenic flux: (I) local etching by the metallic droplet, (II) GaAs growth by consumption of the Ga droplet under As supplied, and (III) evolution of nanoholes to shallower structures. In this picture, the key role of arsenic flux would be the reactivation of dissolution of the GaAs substrate by the metallic Ga droplets and further GaAs growth, processes that are also in the origin of the well-known flat depressions beneath the Ga droplets in the absence of an arsenic flux. Actuation on the kinetics of the processes involved in nanohole formation may facilitate obtaining nanoholes under design, which ultimately will influence the optical properties of the nanostructures formed inside. Acknowledgements We want to acknowledge the financial support from the Spanish MINECO through grants TEC2011-29120-C05-01/04, ENE2012-37804-C02-02, and AIC-B-2011-0806. We also want to acknowledge Raquel Álvaro from the Micro- and Nano-fabrication service (MiNa) at IMM for the AFM measurements.

While this finding was statistically significant only in the multivariate analysis, this program improved quality of antimicrobial utilization and follow-up. Interestingly, the subgroup analysis in the uninsured population suggests that this intervention

could have a dramatic impact in populations with limited access to care. Other characteristics found to be associated with improved outcome were documented urinary frequency and dysuria; the authors speculate that this may be related to improved selleck screening library awareness and aggressive antimicrobial therapy among ED providers responding to these well-defined symptoms of urinary tract infections. In addition, the authors noted a numerical increase in appropriate empiric therapy and a significant increase in the use of nitrofurantoin in the CFU group, corresponding to a change in national and institutional recommendations for cystitis [20]. Despite this, intervention by the multidisciplinary CFU providers was still necessary in 25.5% of cases, and the most common reason for intervention was pathogen non-susceptibility. This is similar to

reports from antimicrobial stewardship programs in other EDs with intervention rates ranging from 15 to 25% [15, 16]. This variance may be due in part to the population selleck chemical that each institution chooses to target. Whilst the authors limited their intervention to urine and blood cultures, others have also included sexually transmitted diseases, skin and skin structure BAY 11-7082 cell line infection, and respiratory tract infections. There are potential limitations to this study that must be considered. The multidisciplinary CFU was only available for culture follow-up Monday–Friday. During weekend shifts, prescribers Sclareol were instructed

to continue culture follow-up with their same pre-intervention method; in nearly all cases this resulted in delaying intervention until the pharmacist initiated follow-up on Monday. Another limitation was reliance on electronic physician documentation to confirm if the patient was reached for changes in therapy. Calculating the time to appropriate therapy was, therefore, based on the day the physician contacted the patient. Limitations may also exist due to the quasi-experimental design, including potential bias in the assessment of empiric appropriate treatment, the lack of study group randomization, and potential for regression toward the mean in the post-intervention group [21]. A quasi-experimental design was selected for the study because withholding multidisciplinary follow-up from randomly selected patients would be impractical and potentially unethical.

5 V. It seems that the resistive switching GSK-3 inhibitor memory device can be programmed under positive voltage through Cu pillar; however, it is not possible to erase through Cu pillar if it needs lower voltage than that of −1.5 V. Further study is needed to improve Cu pillar robustness under negative voltage on the Cu electrode. Figure 7 Data retention and read endurance characteristics. (a) Typical data retention characteristics

of our Al/Cu/Al2O3/TiN CBRAM device. The thickness of Al2O3 layer is 10 nm. (b) Read endurance characteristics of the Cu pillars in a Al/Cu/Al2O3/TiN structure at high CC of 70 mA. The stronger Cu pillars are obtained when the bias is positive. Conclusions The Cu pillars are formed in Al/Cu/Al2O3/TiN Selleckchem Luminespib structure under a small voltage of <5 V and a high current of 70 mA. Tight distribution of robust Cu pillars for 100 randomly measured devices with an average current of approximately 50 mA at a V read of 1 V is observed.

The Cu pillars have long read pulse endurance of >106 cycles under positive read voltage. Although, the read pulse endurance under negative read voltage is worst due Selleckchem EGFR inhibitor to Cu dissolution partially. On the other hand, our Al/Cu/Al2O3/TiN memory device shows good bipolar resistive switching behavior at a CC of 500 μA. Good data retention characteristics of >103 s with acceptable resistance ratio of >10 is observed. It is expected that this novel idea to achieve high-density memory through 3D interconnect will have a good alternative of traditional TSV technique owing to a low cost and simple way. Acknowledgments This work was supported by National Science Council (NSC), Taiwan, under contract no. NSC-102-2221-E-182-057-MY2. The authors are grateful to Electronics and Optoelectronics Research Laboratories Parvulin (EOL)/Industrial Technology Research Institute (ITRI), Hsinchu, for their support. References 1. Prakash A, Jana D, Maikap S: TaO x based resistive switching

memories: prospective and challenges. Nanoscale Res Lett 2013, 8:418.CrossRef 2. Yang JJ, Strukov DB, Stewart DR: Memristive devices for computing. Nat Nanotechnol 2013, 8:13.CrossRef 3. Torrezan AC, Strachan JP, Medeiros-Ribeiro G, Williams RS: Sub-nanosecond switching of a tantalum oxide memristor. Nanotechnology 2011, 22:485203.CrossRef 4. Lee HY, Chen PS, Wu TY, Chen YS, Wang CC, Tzeng PJ, Lin CH, Chen F, Lien CH, Tsai MJ: Low power and high speed bipolar switching with a thin reactive Ti buffer layer in robust HfO 2 based RRAM. Tech Dig Int Electron Devices Meet 2008, 1–4. 5. Chen YS, Lee HY, Chen PS, Liu WH, Wang SM, Gu PY, Hsu YY, Tsai CH, Chen WS, Chen F, Tsai MJ, Lien C: Robust high-resistance state and improved endurance of HfO x resistive memory by suppression of current overshoot. IEEE Electron Device Lett 2011, 32:1585.CrossRef 6. Tsuji Y, Sakamoto T, Banno N, Hada H, Aono M: Off-state and turn-on characteristics of solid electrolyte switch.

Second, male gender, age group, presence of illness, and shift/night selleck work were background risk factors associated with high WRSP prevalence. Third, the overall prevalence of WRSP was 5.1 % in this

population. Although the results must be interpreted with caution because of the cross-sectional nature of the study design, the analyses of this large population-based representative survey suggest that work organization factors are important risk factors for WRSP among Korean workers. Those who experienced sexual harassment at work had a 3.5 times higher risk of WRSP compared to those who had not experienced sexual harassment at work. Although we could not locate studies specifically focused on a relationship

between sexual harassment and workers’ sleep problems, several studies have reported the relationship between Adriamycin sexual harassment and workers’ physical and mental health. A study on female flight attendants showed that for those who experienced sexual harassment, the risk of poor self-rated health was 2.8 times higher than for those who had not had such an experience (Ballard et al. 2006). There are also reports that sexual harassment heightens the risk of depression, somatic symptoms, posttraumatic stress disorder (PTSD), and other medical conditions (Street et al. 2008), which could relate to sleep problems. Sexual harassment also raises the risk of the victims’ harmful alcohol use (Gradus et al. 2008). Given such evidence, workers who experienced sexual harassment may have an increased risk for suffering sleep problems. This study found that the participants who perceived sex-

and age-related discrimination had more than twice the risk of WRSP than those workers who did not. Discrimination is a crucial social issue not only in multiethnic nations such as the United States but also in AZD3965 in vivo non-multiethnic nations as well. In the United States, the occurrence of perceived discrimination over one’s lifetime is 33.5 %, but the prevalence differs greatly by racial/ethnic group; for non-Hispanic whites, it is 30.9 %, for non-Hispanic blacks, 48.9 %, and for other racial/ethnic groups, 50.2 % (Kessler et al. 1999). The results of the 1977–1989 US Longitudinal Survey of Mature Guanylate cyclase 2C Women (n = 1,778) indicated that perceived workplace discrimination ranged between 11.11 and 15.14 % in black women, while it ranged between 12.10 and 16.03 % in white women. Workplace discrimination was found to be one of the strongest predictors for emotional distress and functional limitation (Pavalko et al. 2003). In the current study, the occurrence of age and sex discrimination at the workplace was 3.4 and 1.4 %, respectively, which was lower than those of studies conducted in the United States (Kessler et al. 1999; Pavalko et al. 2003), but the impact on sleep seems substantial.

Cycle parameters were: initial denaturation at 92°C, 5 min; 35 cycles of denaturation at 92°C for 30s, annealing for 1 min, and extension for 1 min at 72°C; 7 min final extension; storage at 4°C. Amplification products were visualized by agarose gel electrophoresis and ethidium bromide staining. One gene pair, cj1318 and cj1336, had extensive overlapping regions of DNA sequence identity. The primers obtained could not differentiate the two genes; for the purposes of our discussion, positive results were

taken to mean that both loci were present, though this has not been unambiguously demonstrated. PCR was undertaken to detect the CJIE1 prophage and ORF11 from CJIE1. The PCR BYL719 ic50 reaction primers and conditions have been described MM-102 solubility dmso previously [6]. An amplification product of approximately 750 bp signified the presence of the CJIE1 prophage while a larger amplification MK-0457 molecular weight product of approximately 1700 bp indicated the presence of the ORF11 indel. A total of 496 Campylobacter spp. isolates

were tested using this PCR method. Adherence and invasion assays Assays were done according to the methods of Malik-Kale et al. [26], except that wells were seeded with 2 × 107 INT-407 cells the day before the assay to give a newly confluent monolayer at the time the assay began. Two strategies were used to perform the adherence and invasion assays. In the first series of experiments only two C. jejuni test isolates were assessed in each experiment along with the C. jejuni 81–176 and E. coli Top 10 control strains. This was done in order to manage the timing of steps and reduce the possibility of technical errors. Almost all of these experiments were done by a single technologist and the INT-407 cells used were between passages 65 – 120. Furthermore, a gentamicin concentration of 750

μg/ml was used to kill extracellular bacteria. A second series of experiments was done to compare the adherence and invasion of all isolates and controls in a single experiment. Fresh INT-407 cells were Dolutegravir obtained and used between passages 5 – 20. For these later experiments, the concentration of gentamicin was reduced to 500 μg/ml based on testing of the strains used. There were no obvious differences in results using either concentration of antibiotic. Results from all assays were used to create Figure 2 and perform the statistical analyses. Similarly, results from the second series of experiments were summarized in Table 2 to show the variability between experiments and common trends when comparing isolates carrying the CJIE1 prophages versus the isolate without the prophage. Values for percent adherence (%A) and percent internalization divided by adherence (%I/A) were described previously [26]. The value for percent adherent was obtained from by dividing the values obtained for adherent bacteria (cfu/ml) by the values obtained for input bacteria (cfu/ml) and multiplying by 100.

Lutra 48(2):91–108 van Wieren SE, Worm PB (2001) The use of a motorway wildlife overpass by large mammals. Neth J Zool 51:97–105 Vos CC, Antonisse-De Jong AG, Goedhart PW, Smulders MJM (2001) Genetic similarity as a measure for connectivity between fragmented populations of the moor frog (Rana arvalis). Repotrectinib concentration Hered 86:598–608CrossRef Yanes M, Velasco J, Suarez F (1995) Permeability of roads and railways to vertebrates:

the importance of culverts. Biol Conserv 71:217–222CrossRef”
“Introduction We define our domain of interest as being those areas of Africa that receive between 300 and 1,500 mm of rain annually. This broad and inevitably arbitrary definition encompasses a wide variety of habitats including grasslands, wetlands, dry woodlands and mosaics of all of these, but most of this area is deemed to be savannah. For our purposes we call all these areas “savannahs” for simplicity, without wishing to comment on the complexities of what determines CBL0137 ic50 the limits of this biome (Sankaran et al. 2005; Ratnam et al. 2011; Staver et al. 2011). Thus defined, we show below that savannahs comprise 13.5 million km2. (This compares

to Cahoon et al.’s (1992) estimate of ~10 million km2.) As we define it, this domain is most of Africa south of the Sahara, excluding the tropical moist forests of West Africa, the Congo, patches of montane forests throughout East Africa, and drier areas in the Southwest, such as the Namib. As such, the IUCN Red List entry (henceforth Bauer et al. 2008) shows that savannah Africa encompasses

most of the present range of the African lion (Panthera leo leo). Lions once lived across Eurasia, but now only a remnant population of a different subspecies (Panthera leo persica) survives in India. Recent research has demonstrated that the lion in West and Central Africa is genetically different from the lion in East and TGF-beta inhibitor Southern Africa and more closely resembles Asiatic populations (Bertola et al. 2011). Nonetheless, we consider just African populations and do so without distinction. In Africa, lion populations once lived outside this strict savannah zone. For example, until recently a lion population was present in forest-savannah mosaics in Gabon and the Republic of Congo (“Congo-Brazzaville”) (Henschel 2009), and there are other remnant populations Venetoclax price in forests in Ethiopia (see supplemental materials) and other non-savannah environments. However, the association between lions and savannahs is generally now quite a close one. How much of the African savannah still supports lions—and is likely to do so in the future—are the more difficult questions we address in this paper. We evaluate the state of the African savannah with two objectives, namely estimating the areas of savannah still suitable for lion populations and estimating the lion populations themselves within these areas.

The solving of ITE in terms of the five-parametric models that takes into account the presence in the sample of both absorption and non-uniformity (sharp or smooth) showed the more adequate character of the model with sharp non-uniformity: Lower subscripts denote the following: l, lower; u, upper. Note that in terms of both of these models, the n value of oxide Selleck 3-MA film is below 1.46. It may be due to the appearance of porosity in the oxide film and/or change of its composition through the partial replacement of silicon atoms by carbon atoms. The complication of the two-layer model by introducing birefringence, dichroism, non-uniformity in both lower and upper layers did not lead to any noticeable reduction

of MSEmin, despite the fact that the number of Avapritinib research buy variable parameters increased to 8. The obtained AZD5582 values of the parameters describing the deviation of these models from the ‘lower IUTL – upper IUAL’ model were small in this case. This indicates the sufficient adequacy of

the ‘lower IUTL – upper IUAL’ model. Let us turn to the values of the optical constants of thin upper film. Its refractive index value (3.24) is higher and absorption index value (0.463) is lower than the reported values for bulk graphite, the film consisting of 8 to 9 graphene layers, and single-layer graphene (n = 2.73, k = 1.42 are found at λ = 633 nm for bulk graphite [16]; n = 2.68, k = 1.24 at λ = 633 nm are found for the film consisting of 8 to 9 layers of graphene [17]; n = 2.7 to 2.8, k = 1.4 to 1.6 [18] and n = 2.5 to 2.7, k = 1.1 to 1.4 [19] have been reported for single-layer graphene). On the other hand, these values are very Glycogen branching enzyme close to the values of the optical constants for a-C films deposited using pulsed laser deposition (n ~ 3.10, k ~ 0.40 at λ = 633 nm) [20]. Also, the value of Imϵ = 2 × 3.24 × 0.463 = 3.00 calculated based upon our data is in the middle

of the range for the values Imϵ = 2.0 to 4.0. This range has been previously obtained at λ = 633 nm for laser-irradiated carbon films with a large amount of graphite phase and dominating sp 2-type bonds [21]. Thus, from the ellipsometric analysis, it follows that as a whole, the upper film can be treated as a disordered graphite-like layer having the thickness approximately equal to three-layer graphene. This result proves the realization of the first scenario among those that are compatible with XPS measurements. Weak intensity as well as unstructured micro-Raman spectra in most of the measured points of the type II sample indicates the formation of the strongly disordered amorphous carbon-based phase with large number of defects. (Similar character of the Raman spectra had been observed, for example, in the carbon films obtained by the electron-beam-induced high-speed evaporation of graphite on substrates preheated to 700°C to 800°C [22]).

Measurements were made before and after

(0, 24, 48 and 72 h) 120 minutes of treadmill walking at 6.5 km·h-1 (n = 10) on a level gradient (0%) carrying a 25 kg backpack with GDC-0449 supplier consumption of 250 ml (at 0 and 60 minutes) of a beverage containing either placebo (PLA – Black square), carbohydrate (6.4%) (CHO – Black triangle) or protein (7%) (PRO – Black circle) and twice daily (500 ml, morning and evening) for the 3 days after load carriage (n = 10). Symbols show difference from pre measurement for PLA (* P < 0.05), CHO († P < 0.05), PRO (# P < 0.05). Isokinetic Contractions of the Shoulder Extensors and Flexors There were no changes over time in any condition for the shoulder extensors (60°·s-1) (P = 0.124), shoulder extensors (180°·s-1) (P = 0.101), shoulder flexors (60°·s-1) (P = 0.094) or shoulder flexors (180°·s-1) (P = 0.078). VX-689 ic50 Discussion The primary finding of the present study was that time course of recovery of neuromuscular function following prolonged load carriage (2 h, 25 kg) is improved with consumption of whey protein and carbohydrate beverages. After load carriage, isometric knee extension force recovered to pre-exercise values following 48 h recovery with carbohydrate and whey protein beverages compared to 72 h recovery

with a placebo. Interestingly, recovery of isokinetic peak torque was not improved by supplementation. However, our experimental model had similar absolute loads during load carriage that may have resulted in large variation. It is possible that this large variation and our choice of analysing different recovery time points has masked, for example, potential improved effects of both supplements at 48 h for peak torque (60°·s-1) of the knee extensors (Figure 2) and the effect of whey protein at 48 h for peak torque (60°·s-1) of knee flexors (Figure 3). Reductions in torque in the present study are supported by data of Clarke et al. [1], which showed decreases in strength of knee and trunk extensors and flexors after a

12.1 km road march at 4 km·h-1 carrying a 27 kg load. Clarke et al. [1] find more observed larger Casein kinase 1 decreases in knee extensor peak torque (6 vs. 8%) but smaller decreases in knee flexor peak torque (9 vs. 6%) with comparable reductions for changes in trunk extensor (12 vs. 11%) and flexor peak torque (10 vs. 11%). Whey protein intake during resistance training has been shown to improve muscle hypertrophy [7] and maintain a positive protein balance [15]. The effect of whey protein supplementation on recovery of muscle function after resistance or endurance exercise has received little attention. Buckley et al. [16] observed a ~23% decrease in isometric force of the knee extensors after 100 maximal eccentric contractions.

Curcumin, a naturally occurring flavinoid and proapoptotic compound derived from the rhizome of Curcuma longa, has strong anti-inflammatory, antioxidant, anticarcinogen, anticancer properties check details through regulating multiple downstream cancer-related signaling molecules. The molecular targets of curcumin include modulation of NF-kappaB, Jak/STAT, WT1, extracellular signal regulated kinase and other key molecules involved

in tumorigenesis [6–8]. The mechanisms underlying the anticancer activity of curcumin have been widely investigated. Bharti et al. showed curcumin decreased NF-kappaB in human multiple myeloid cells, leading to the suppression of proliferation and induction of apoptosis [7]. Recently more and more data have shown that WT1 is a very important target gene by curcumin [9]. However the exact mechanism by which curcumin downregulated the expression of WT1 is still not clear. MicroRNAs (miRNAs) are non-coding regulatory RNAs of 21 to 25

nucleotides which regulate most of basal progress such as cell proliferation, survival, apoptosis, and Momelotinib nmr differentiation by triggering either translational repression or mRNA degradation [10]. Furthermore, computational prediction demonstrated that each miRNA may target hundreds of genes, and that more than 50% of human protein-coding genes could be modulated by miRNAs [11]. Recently some data have indicated pure curcumin inhibited cancer cell proliferation though miRNAs mediated signal pathway. Michael et al. showed curcumin inhibited the proliferation of pancreatic cancer cells through upregulation of miR-22 and downregulation learn more of miR-199a* [12]. Yang et al. demonstrated that curcumin induced MCF-7 cells apoptosis through miR-15a/16-1 mediated down-regulation of Bcl-2 [13]. These emerging results suggest that specific targeting of miRNAs by natural agents may open new avenues for the complete elucidation of antitumor activity by curcumin. In this study, we explored the potential modulation of miR-15a and miR-16-1

by curcumin in leukemic cells. Our study aims to explain a new mechanism by which curcumin downregulates the expression of WT1 via the upregulation of miR-15a/16-1 in leukemic P-type ATPase cells. Material and methods Cell lines and primary AML cells Leukemic cell lines (K562 and HL-60) were employed for the present study. All cells were cultured in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum (Invitrogen, CA, USA) in humidified 37°C incubator with 5% CO2. Primary leukemic cells were obtained from 12 patients with acute myeloid leukemia (AML) (3 M2, 2 M3, 3 M4 and 4 M5, The First Affiliated Hospital of Wenzhou Medical College) with informed consent. The detailed data of the patients were showed in Table 1. The diagnosis was established according to French-American-British classification. All manipulations were approved by the Medical Science Ethic Committee of Wenzhou Medical College.